Journal: Life Science Alliance

Article Title: Defects in integrin complex formation promote CHKB -mediated muscular dystrophy

doi: 10.26508/lsa.202301956

Figure Lengend Snippet: (A, B) Western blot of total tissue lysis of (A) forelimb (triceps) and (B) hindlimb (quadriceps) samples from three distinct (lanes 1–3) Chkb +/+ and three distinct (lanes 4–6) Chkb −/− mice probed with anti-vinculin, anti-itga7, anti-talin, anti-α-actinin, and anti-Gapdh antibodies. Bottom: densitometry of the Western blot data show the ratio of vinculin, metavinculin, itga7, talin, and a-actinin to Gapdh in forelimb (triceps) and hindlimb (quadriceps) samples. Values are means ± SD; n = 3–4 per group. * P < 0.05, ** P < 0.01, *** P < 0.001 ( t test). Detergent (NP40), soluble (cytosolic).

Article Snippet: The following antibodies were used: Vinculin (1:1,000, Cat#ab88053; Abcam), Itga7 (1:1,000, Cat#Ab24509; Abcam), Talin (1:1,000, Cat#AHP1272; Bio-Rad), α-actinin (1:1,000, Cat#22170-1-AP; Proteintech), Chkβ (1:250, Cat#398957; Santa Cruz), GAPDH (1:1,000, Cat#398957; Cell signaling).

Techniques: Western Blot, Lysis

Journal: Life Science Alliance

Article Title: Defects in integrin complex formation promote CHKB -mediated muscular dystrophy

doi: 10.26508/lsa.202301956

Figure Lengend Snippet: (A, B) Soluble (A) and insoluble (sarcolemma/cytoskeleton associated) (B) fractions prepared from hindlimb skeletal muscle (quadriceps) of three distinct (lanes 1–3) Chkb +/+ and three distinct (lanes 4–6) Chkb −/− mice probed with anti-vinculin, anti-talin, anti-α-actinin, and anti-β-actin. (C) Densitometry of the Western blot data show the ratio of detergent insoluble to detergent soluble fractions of vinculin, β-actin, and α-actinin in hindlimb (quadriceps) samples. Much less vinculin, β-actin, and α-actinin was associated with the cytoskeleton in lysates from Chkb −/− , compared with Chkb +/+ mice. Values are means ± SD; n = 3–4 per group. * P < 0.05, ** P < 0.01, *** P < 0.001 ( t test).

Article Snippet: The following antibodies were used: Vinculin (1:1,000, Cat#ab88053; Abcam), Itga7 (1:1,000, Cat#Ab24509; Abcam), Talin (1:1,000, Cat#AHP1272; Bio-Rad), α-actinin (1:1,000, Cat#22170-1-AP; Proteintech), Chkβ (1:250, Cat#398957; Santa Cruz), GAPDH (1:1,000, Cat#398957; Cell signaling).

Techniques: Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of curcumin on the apoptosis of cardiomyocytes and the expression of NF-κB, PPAR-γ and Bcl-2 in rats with myocardial infarction injury

doi: 10.3892/etm.2016.3858

Figure Lengend Snippet: Optical density of NF-κB and PPAR-γ protein expression in the cardiomyocytes of rats (mean ± standard deviation).

Article Snippet: Following blocking with 10% goat serum at 37°C for 15 min, the slices were incubated with rabbit anti-PPAR-γ polyclonal antibody (Peprotech Inc., Rocky Hill, NJ, USA; 1:200) or rabbit anti-NF-κBp6 polyclonal antibody (Peprotech Inc.; 1:100) at 4°C overnight.

Techniques: Expressing, Control

Journal: Nutrition Research and Practice

Article Title: Effects of d-α-tocopherol supplements on lipid metabolism in a high-fat diet-fed animal model

doi: 10.4162/nrp.2013.7.6.481

Figure Lengend Snippet: Expression of (A) PPAR-α and (B) PPAR-γ in the liver. CON; mice fed regular diet (10% of calories derived from fat) with distilled water as a vehicle (0.1 ml, p.o. ) for 20 weeks. HF; mice fed high-fat diet (45% of calories derived from fat) with distilled water as a vehicle (0.1 ml, p.o. ) for 20 weeks. HF-E; mice fed high-fat diet (45% of calories derived from fat) with d-α-tocopherol as a vehicle for 20 weeks. D-α-tocopherol (100 IU/kgBW, p.o. ); freshly prepared by dissolving in distilled water after triturating with Tween 20. PPAR-α; peroxisome proliferator-activated receptor-alpha. PPAR-γ; peroxisome proliferator-activated receptorgamma

Article Snippet: After being transferred, the membranes were blocked overnight in TBS containing Tween 20, then probed with the appropriate primary antibody (rabbit anti-PPAR-γ (1/1000, Cell Signaling Technology, USA) or rabbit anti-PPAR-α (1/1000, Bioworld Technology, USA)).

Techniques: Expressing, Derivative Assay

Journal: PPAR Research

Article Title: Ligand-Activated Peroxisome Proliferator-Activated Receptor β / δ Facilitates Cell Proliferation in Human Cholesteatoma Keratinocytes

doi: 10.1155/2020/8864813

Figure Lengend Snippet: Immunohistochemical staining for proliferator-activated receptor β / δ . PPAR β / δ is scantily expressed in external auditory canal skin (a–c). PPAR β / δ is expressed in the cells mainly in the parabasal and basal layers of cholesteatoma epithelium (d–f). The intensity of its expression is decreased in the granular and prickle cell layers (magnification, ×200).

Article Snippet: After a brief rinse, the sections were incubated overnight with rabbit polyclonal anti-PPAR β / δ antibody (Genetex, Cambridge, MA, USA) at 250-fold dilution.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: PPAR Research

Article Title: Ligand-Activated Peroxisome Proliferator-Activated Receptor β / δ Facilitates Cell Proliferation in Human Cholesteatoma Keratinocytes

doi: 10.1155/2020/8864813

Figure Lengend Snippet: Ligand activation of PPAR β / δ facilitates the proliferation of human cholesteatoma keratinocytes in vitro. (a, c) The effect of GW0742 (a high affinity PPAR β / δ agonist) (a) and GSK0660 (a high affinity PPAR β / δ antagonist) (c) on cell proliferation was detected by EdU assays. (b, d) Data were based on at least three independent experiments and presented as the mean ± SD; ∗ p < 0.05 (vs. control group).

Article Snippet: After a brief rinse, the sections were incubated overnight with rabbit polyclonal anti-PPAR β / δ antibody (Genetex, Cambridge, MA, USA) at 250-fold dilution.

Techniques: Activation Assay, In Vitro, Control

Journal: PPAR Research

Article Title: Ligand-Activated Peroxisome Proliferator-Activated Receptor β / δ Facilitates Cell Proliferation in Human Cholesteatoma Keratinocytes

doi: 10.1155/2020/8864813

Figure Lengend Snippet: Expression of PPAR β / δ and ligand activation of target gene (PDK1) in cholesteatoma keratinocytes. (a, c) Expression of PPAR β / δ was quantified by immunoblot after treating with 100 nM GW0742 (a) or 5 μ M GSK0660 (c) for 24 h. (e, g) The effect of PPAR β / δ agonist (e) or antagonist (g) on expression of the PPAR β / δ -dependent target gene PDK1 was determined by immunoblot following ligand activation of PPAR β / δ with 100 nM GW0742 or 5 μ M GSK0660 for 24 h. (b, d, f, and h) Data are expressed as the mean ± SD of the mean for experiments run in triplicate. ∗ p < 0.05 (vs. control group).

Article Snippet: After a brief rinse, the sections were incubated overnight with rabbit polyclonal anti-PPAR β / δ antibody (Genetex, Cambridge, MA, USA) at 250-fold dilution.

Techniques: Expressing, Activation Assay, Western Blot, Control

Journal: PPAR Research

Article Title: Ligand-Activated Peroxisome Proliferator-Activated Receptor β / δ Facilitates Cell Proliferation in Human Cholesteatoma Keratinocytes

doi: 10.1155/2020/8864813

Figure Lengend Snippet: Ligand activation of PPAR β / δ had effect on PDK1/PTEN/AKT/GSK3 β /Cyclin D1 signal pathway in cultured cholesteatoma keratinocytes. (a) Western blot showing changes in PDK1/PTEN/AKT/GSK3 β /Cyclin D1 pathway after treating with 100 nM GW0742 for 24 h. (b) Immunoblots demonstrating the effect of GSK0660 treatment (5 μ M, 24 h) on the PDK1/PTEN/AKT/GSK3 β /Cyclin D1 pathway. β -Actin served as loading control. ∗ p < 0.05.

Article Snippet: After a brief rinse, the sections were incubated overnight with rabbit polyclonal anti-PPAR β / δ antibody (Genetex, Cambridge, MA, USA) at 250-fold dilution.

Techniques: Activation Assay, Cell Culture, Western Blot, Control